cdx2 cre  (Jackson Laboratory)

Journal: Disease Models & Mechanisms

Article Title: Chiari II brain malformation is secondary to open spina bifida

doi: 10.1242/dmm.052528

Figure Lengend Snippet: Generation of the Cdx2 cre/+ ;Pax3 fl/fl mouse model. (A) Genetic cross used to generate mice with spina bifida (SB). Cdx2 cre drives Pax3 knockout in the body only (light-green highlight), whereas the head remains wild type. See Table 1 for offspring genotypes. (B,C) Cdx2 cre driving mTmG reporter expression in the body at embryonic day (E)10.5 (B; blue) and E15.5 (C; green). Magenta in B indicates a region of no Cdx2 cre -mediated recombination. Dashed line in C indicates outline of the fetus. See also Fig. S1 . (D,E) Representative images of E15.5 control ( Cdx2 +/+ ;Pax3 fl/fl ; D) and mutant ( Cdx2 cre/+ ;Pax3 fl/fl ; E) fetuses. White arrowheads indicate the location of the open SB lesion (in E). See also Fig. S2 . (F) Phenotypic developmental timeline of Cdx2 cre/+ ;Pax3 fl/fl embryos and fetuses, with dashed lines indicating the extent of open SB lesions. Scale bars: 1.0 mm.

Article Snippet: Cdx2 cre/+ male mice ( ) were obtained from The Jackson Laboratory [JAX; strain 009350 : B6.Cg-Tg(CDX2-cre)101Erf/J] and maintained by breeding with C57BL/6J females.

Techniques: Knock-Out, Expressing, Control, Mutagenesis

Journal: Disease Models & Mechanisms

Article Title: Chiari II brain malformation is secondary to open spina bifida

doi: 10.1242/dmm.052528

Figure Lengend Snippet: PAX3 expression in E10.5 control and Cdx2 cre/+ ;Pax3 fl/fl embryos. Sagittal cryosections of control ( n =3) and SB ( n =3) embryos, immunostained for PAX3 (yellow) and nuclear stained with DAPI (magenta). (A,B) Head sections: PAX3 expression is present in the neural tube (hindbrain, outlined by dashed lines) in both control (A) and SB (B) embryos (yellow arrowheads). (C,D) Lower body: PAX3 expression is present in the dorsal neural tube and dermomyotomes of the control spinal region (yellow arrowheads in C), but is not detectable in the SB embryo (D), consistent with knockout of Pax3 in the lower body. Bracket in D indicates spina bifida region. Scale bar: 0.5 mm.

Article Snippet: Cdx2 cre/+ male mice ( ) were obtained from The Jackson Laboratory [JAX; strain 009350 : B6.Cg-Tg(CDX2-cre)101Erf/J] and maintained by breeding with C57BL/6J females.

Techniques: Expressing, Control, Staining, Knock-Out

Journal: Cell reports

Article Title: The dorsal column nuclei scale mechanical sensitivity in naive and neuropathic pain states

doi: 10.1016/j.celrep.2025.115556

Figure Lengend Snippet: (A) The gracile nucleus (Gr, green) receives sensory inputs from lower body DRG afferents (cyan) and spinal projections (magenta). Retrograde virus injections into the VPL (right) labeled Gr VPL-projecting neurons (PNs). (B) Injection of cholera toxin β subunit (CTb, cyan) into the hindpaw glabrous skin labeled hindlimb afferents targeting the Gr (white dashed circle). Scale bar, 200 μm. (C) Advillin Cre ;Rosa26 LSL-Synaptophysin-GFP mice labeled synaptic terminals of primary afferents (cyan) in the Gr core (solid white line) and shell (dashed white line). Scale bar, 100 μm. (D) Cdx2 Cre ;Lbx1 FlpO ;Rosa26 LSL-FSF-Synaptophysin-GFP mice labeled terminals of spinal projections (magenta) in the Gr core (solid white line) and shell (dashed white line). Scale bar, 100 μm. (E) In situ hybridization of excitatory (vGluT2, blue) and inhibitory (vGAT, red; GlyT2, green) neurons in the Gr core (solid white line) and shell (dashed white line). Scale bar, 100 μm. (F and G) Quantification of excitatory and inhibitory neuronal markers in the Gr core (F) and shell (G). (H) Strategy to label VPL-PNs (green) or inhibitory neurons (red). Inset: representative injection of AAV-retro GFP into the VPL. Scale bar, 1,000 μm. (I and J) Representative images of Gr VPL-PNs (I) (green), inhibitory neurons (J) (red), and NeuN + immunostaining of neurons (blue). Scale bar, 100 μm. (K and L) Quantification of VPL-PNs and inhibitory neurons in the Gr core (K) and shell (L) normalized to total NeuN.

Article Snippet: Mouse: Cdx2 Cre , Jackson Laboratories , Jax#009350.

Techniques: Virus, Labeling, Injection, In Situ Hybridization, Immunostaining

Journal: bioRxiv

Article Title: Chiari II brain malformation is secondary to open spina bifida

doi: 10.1101/2025.01.06.631442

Figure Lengend Snippet: ( A ) Illustration of the Cre-LoxP cross used to generate mice with SB phenotype. Cdx2Cre drives Pax3 knockout in the body only (light green highlight). The head remains genetically wild-type. ( B, C ) Cdx2Cre driving mTmG reporter expression in the body of E10.5 (A, cyan) and E15.5 (B, green). Magenta in (B) indicates region of no Cdx2 expression. ( D, E ) Representative images of E15.5 control (non-Cre; Pax3 fl/fl ) and mutant ( Cdx2 cre ;Pax3 flfl ) embryos. White arrows indicate the location of the open SB lesion (in E). ( F ) Phenotypic developmental timeline of Cdx2 cre ;Pax3 flfl mice, with dotted line indicating extent of open AV lesion. All scale bars = 500 µm.

Article Snippet: Cdx2 Cre male mice were obtained from the Jackson Laboratory (strain 009350: B6.Cg-Tg(CDX2-cre)101Erf/J) and maintained by breeding with C57BL/6J females.

Techniques: Knock-Out, Expressing, Control, Mutagenesis

Journal: bioRxiv

Article Title: Chiari II brain malformation is secondary to open spina bifida

doi: 10.1101/2025.01.06.631442

Figure Lengend Snippet: Sagittal cryosections of control (n = 3) and SB (n = 3) embryos, immunostained for Pax3 (yellow) and nuclear-stained with DAPI (magenta). ( A, B ) Head sections: Pax3 expression is present in the neural tube (rhombencephalon) of both control and SB (yellow arrowheads). ( C, D ) Lower body: Pax3 expression is present in the spinal region of control (yellow arrowheads), but it is not detectable in the SB section, consistent with lower body Pax3 knockout. ( E ) The four genotypes resulting from the mating in , with % values from 10 litters, and number of embryos with normal or SB phenotypes. Note the exclusive occurrence and full penetrance of SB in Cdx2 cre ;Pax3 flfl embryos. Overall genotype ratios do not differ significantly from Mendelian expectation (p > 0.05). Scale bar = 500 µm for A-D.

Article Snippet: Cdx2 Cre male mice were obtained from the Jackson Laboratory (strain 009350: B6.Cg-Tg(CDX2-cre)101Erf/J) and maintained by breeding with C57BL/6J females.

Techniques: Control, Staining, Expressing, Knock-Out

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Colorectal 15-Lipoxygenase-1 as a Host Factor Determining the Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Colorectal Tumorigenesis in Mice

doi: 10.1016/j.jcmgh.2025.101607

Figure Lengend Snippet: Generation and characterization of a conditional ALOX15 mouse model (iALOX15). ( A ) Schematic for the generation of iALOX15. Two mouse models could be generated from one construct as shown: the first allowing inducible ALOX15 expression driven by the CAG promoter, and the second was driven by the mouse Rosa26 promoter. The genuine Rosa26 promoter is further upstream, and once in embryonic stem (ES) cells, its transcript is spliced into the splice acceptor of exon 2 (“s.acc.”, purple ). In the native state, transcription is aborted at the insulator site (H19, black ), and instead starts at the CAG promoter (pCAG, yellow arrow ). The loxP-flanked (lox, blue arrow heads ) neo–STOP cassette for conditional activation has a stop site that is a large region of the SV40 intron plus late poly A (neo, green arrow ; STOP, blue box ). The ALOX15 cDNA is inserted downstream of the neo/stop cassette and is followed by an IRES-driven eGFP reporter gene. For exchange of the promoters, the CAG-cassette is flanked by FRT-sites. ( B ) ALOX15 expression of the three selected ES cell clones infected with or without Cre-recombinase expressing adenovirus. Three clones of ES cells (A4, E12, and G2) were cultured and infected with Cre-recombinase (Cre) expressing adenovirus (MOI = 200) for 24 hours. ALOX15 expression was measured by Western blot. ( C, D ) Generation and genotyping of iALOX15 chimeric mice. Two ES cell clones (A4, E12) were injected into blastocysts from C57BL/6 albino mice. Three of the living pups being 30% to 70% chimeric (black on white) were derived from ES cell clone A4 ( C ). ALOX15 cDNA expression level of genomic DNA was measured by qPCR. ∗∗∗∗ P < .0001 compared with C57BL/6 WT (WT: negative control) ( D ). ( E–G ) Characterization of the new iALOX15 mouse model. iALOX15 mice with Rosa26 promoter were bred with either CDX-Cre (mouse #1 to #4) or Villin-Cre mice (mouse #5 to #10) to induce ALOX15 expression via targeted intestinal Cre recombinase expression. ( E ) Colonic ALOX15 expression was observed in colonic samples from mice that carried both iALOX15 and Cre transgenic genes (mouse #3 and #4 [iALOX15;CDX-Cre], mouse #6 and #10 [iALOX15;Villin-Cre]) but not in those that just carried iALOX15 alone (mouse #1 and #8) or Cre gene alone (mouse #2 [CDX2-Cre], mouse #7 and #9 [Villin-Cre]) or none of them [wild-type (WT)] (mouse #5). + represents ALOX15 positive control. ( F ) Colonic ALOX15 expression was enzymatically active, as demonstrated by markedly increased 13-HODE. ( G ) Successful Cre recombinase in iALOX15; VIllin-Cre mice also induced EGFP expression, measured by IF staining. ( H ) Comparative analysis of ALOX15 mRNA expression between human normal colonic endoscopic biopsy samples collected from patients with CRC (N = 8) and colonic mucosa samples of tamoxifen-treated iALOX-15;Villin-CreERT2 mice (generated by breeding Villin-CreERT2 mice with iALOX15 mice) (N = 8). Values are means ± SEM. Two-tailed t -test. ns, not significant.

Article Snippet: Cg-Tg ( CDX2 -Cre) 101Erf/J mice (designated as CDX2-Cre, #009350) and B6.129S2-Alox15 tm1Fun /J homozygous mice (designated as 12/15Lox KO , #002778) were purchased from Jackson Laboratory (Bar Harbor, Maine).

Techniques: Generated, Construct, Expressing, Activation Assay, Clone Assay, Infection, Cell Culture, Western Blot, Injection, Derivative Assay, Negative Control, Transgenic Assay, Positive Control, Staining, Two Tailed Test